Isolation and Characterization of a Highly Mutated Chinese Isolate of Enterovirus B84 from a Patient with Acute Flaccid Paralysis.
Sci Rep
; 6: 31059, 2016 08 08.
Article
in En
| MEDLINE
| ID: mdl-27499334
ABSTRACT
Enterovirus B84 (EV-B84) is a newly identified serotype within the species Enterovirus B (EV-B). To date, only ten nucleotide sequences of EV-B84 are published and only one full-length genome sequence (the prototype strain) is available in the GenBank database. Here, a highly mutated EV-B84 (strain AFP452/GD/CHN/2004) was recovered from a patient with acute flaccid paralysis in the Guangdong province of China in 2004 making this the first report of EV-B84 in China. Sequence comparison and phylogenetic dendrogram analysis revealed high variation from the global EV-B84 strains (African and Indian strains) and frequent intertypic recombination in the non-structural protein region, suggesting high genetic diversity in EV-B84. The Chinese EV-B84 strain, apparently evolving independently of the other ten strains, strongly suggests that the EV-B84 strain has been circulating for many years. However, the extremely low isolation rate suggests that it is not a prevalent EV serotype in China or worldwide. This study provides valuable information about the molecular epidemiology of EV-B84 in China, and will be helpful in future studies to understand the association of EV-B84 with neurological disorders; it also helps expand the number of whole virus genome sequences of EV-B84 in the GenBank database.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Paraplegia
/
Enterovirus B, Human
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Enterovirus Infections
Type of study:
Observational_studies
/
Risk_factors_studies
Limits:
Child, preschool
/
Humans
/
Male
Language:
En
Journal:
Sci Rep
Year:
2016
Document type:
Article
Affiliation country: